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Nat Methods. 2017 Jan;14(1):53-56. doi: 10.1038/nmeth.4074. Epub 2016 Nov 21.

mScarlet: a bright monomeric red fluorescent protein for cellular imaging.

Author information

1
Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherlands.
2
European Synchrotron Radiation Facility, Grenoble, France.
3
Institut de Biologie Structurale, Université Grenoble Alpes, CNRS, CEA, Grenoble, F-38044, France.

Abstract

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.

PMID:
27869816
DOI:
10.1038/nmeth.4074
[Indexed for MEDLINE]

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