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Nutrients. 2016 Nov 17;8(11). pii: E733.

An Exploratory Investigation of Endotoxin Levels in Novice Long Distance Triathletes, and the Effects of a Multi-Strain Probiotic/Prebiotic, Antioxidant Intervention.

Author information

1
Department of Life Sciences, Faculty of Science and Technology, Anglia Ruskin University, Cambridge Campus, Cambridge CB1 1PT, UK. justin.roberts@anglia.ac.uk.
2
Department of Life Sciences, Faculty of Science and Technology, Anglia Ruskin University, Cambridge Campus, Cambridge CB1 1PT, UK. craig.suckling@pgr.anglia.ac.uk.
3
Department of Life Sciences, Faculty of Science and Technology, Anglia Ruskin University, Cambridge Campus, Cambridge CB1 1PT, UK. georgia.peedle@student.anglia.ac.uk.
4
College Lane, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK. jamurphy123@gmail.com.
5
Cardiff School of Sport, Cardiff Metropolitan University, Cyncoed Campus, Cyncoed Road, Cardiff CF23 6XD, UK. tdawkins@cardiffmet.ac.uk.
6
College Lane, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK. m.g.roberts@herts.ac.uk.

Abstract

Gastrointestinal (GI) ischemia during exercise is associated with luminal permeability and increased systemic lipopolysaccharides (LPS). This study aimed to assess the impact of a multistrain pro/prebiotic/antioxidant intervention on endotoxin unit levels and GI permeability in recreational athletes. Thirty healthy participants (25 males, 5 females) were randomly assigned either a multistrain pro/prebiotic/antioxidant (LAB⁴ANTI; 30 billion CFU·day-1 containing 10 billion CFU·day-1Lactobacillus acidophilus CUL-60 (NCIMB 30157), 10 billion CFU·day-1Lactobacillus acidophillus CUL-21 (NCIMB 30156), 9.5 billion CFU·day-1Bifidobacterium bifidum CUL-20 (NCIMB 30172) and 0.5 billion CFU·day-1Bifidobacterium animalis subspecies lactis CUL-34 (NCIMB 30153)/55.8 mg·day-1 fructooligosaccharides/ 400 mg·day-1 α-lipoic acid, 600 mg·day-1N-acetyl-carnitine); matched pro/prebiotic (LAB⁴) or placebo (PL) for 12 weeks preceding a long-distance triathlon. Plasma endotoxin units (via Limulus amebocyte lysate chromogenic quantification) and GI permeability (via 5 h urinary lactulose (L): mannitol (M) recovery) were assessed at baseline, pre-race and six days post-race. Endotoxin unit levels were not significantly different between groups at baseline (LAB⁴ANTI: 8.20 ± 1.60 pg·mL-1; LAB⁴: 8.92 ± 1.20 pg·mL-1; PL: 9.72 ± 2.42 pg·mL-1). The use of a 12-week LAB⁴ANTI intervention significantly reduced endotoxin units both pre-race (4.37 ± 0.51 pg·mL-1) and six days post-race (5.18 ± 0.57 pg·mL-1; p = 0.03, ηp² = 0.35), but only six days post-race with LAB⁴ (5.01 ± 0.28 pg·mL-1; p = 0.01, ηp² = 0.43). In contrast, endotoxin units remained unchanged with PL. L:M significantly increased from 0.01 ± 0.01 at baseline to 0.06 ± 0.01 with PL only (p = 0.004, ηp² = 0.51). Mean race times (h:min:s) were not statistically different between groups despite faster times with both pro/prebiotoic groups (LAB⁴ANTI: 13:17:07 ± 0:34:48; LAB⁴: 12:47:13 ± 0:25:06; PL: 14:12:51 ± 0:29:54; p > 0.05). Combined multistrain pro/prebiotic use may reduce endotoxin unit levels, with LAB⁴ANTI potentially conferring an additive effect via combined GI modulation and antioxidant protection.

KEYWORDS:

antioxidants; endotoxemia; prebiotics; probiotics; triathlon

PMID:
27869661
PMCID:
PMC5133117
DOI:
10.3390/nu8110733
[Indexed for MEDLINE]
Free PMC Article

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