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J Gastroenterol Hepatol. 2016 Nov 21. doi: 10.1111/jgh.13664. [Epub ahead of print]

PRDX2 up-regulation in inflammatory bowel disease: friend or foe?

Author information

  • 1Laboratory of Genetics and Molecular pathologies, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, Morocco.
  • 2Laboratory of Thrombosis and Hemostasis, Montreal Heart Institute, Montreal, Quebec, Canada.
  • 3Laboratory of Physiology and Molecular Genetics, Faculty of Sciences Aïn Chock, Hassan II University, Casablanca, Morocco.
  • 4Gastroenterology Department, CHU IbnRochd, Casablanca, Morocco.
  • 5Instituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas, Granada, Spain.

Abstract

BACKGROUND:

Inflammatory bowel diseases (IBD) are chronic multi-factorial inflammatory disorders. Accumulating investigations have provided compelling evidence that describe the interplay of a complex genetic landscape and inappropriate inflammatory response to intestinal microbes in disease etiopathogenesis, but still pose challenges in diagnostic practices.

METHOD:

In this study, comparative proteomic analysis was conducted to identify disease specific proteins underlying IBD pathogenetic mechanisms. Total blood proteins of the IBD patients and healthy subjects were analyzed with one-dimensional electrophoresis; differentially expressed bands were excised and subjected to MALDI-TOF/TOF-MS/MS along with nLC-ESI-MS/MS analysis. Presence of glycosylation, hydroxylation and phosphorylation post-translational modifications was further investigated by immunoprecipitation.

RESULTS:

PRDX2 and Hemoglobin-subunits proteins, which are closely involved in the response to oxidative stress, were identified. PRDX2 was selected for further validation using Western blot and RT-PCR. PRDX2 over-expression was restricted to the protein level within the membrane fraction. Immunoprecipitation identified PRDX2 to be post-translationally glycosylated and phosphorylated.

CONCLUSION:

Our findings demonstrate the implication of PRDX2 in IBD. Future studies are required to establish its functional role and to determine the clinical utility.

KEYWORDS:

Hemoglobin-subunits; IBD; PRDX2

PMID:
27869326
DOI:
10.1111/jgh.13664
[PubMed - as supplied by publisher]
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