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Curr Biol. 2016 Dec 5;26(23):3245-3254. doi: 10.1016/j.cub.2016.10.024. Epub 2016 Nov 17.

SYP73 Anchors the ER to the Actin Cytoskeleton for Maintenance of ER Integrity and Streaming in Arabidopsis.

Author information

1
MSU-DOE Plant Research Lab and Plant Biology Department, Michigan State University, East Lansing, MI 48824, USA.
2
MSU-DOE Plant Research Lab and Plant Biology Department, Michigan State University, East Lansing, MI 48824, USA. Electronic address: fb@msu.edu.

Abstract

The endoplasmic reticulum (ER) is an essential organelle that spreads throughout the cytoplasm as one interconnected network of narrow tubules and dilated cisternae that enclose a single lumen. The ER network undergoes extensive remodeling, which critically depends on membrane-cytoskeleton interactions [1]. In plants, the ER is also highly mobile, and its streaming contributes significantly to the movement of other organelles [2, 3]. The remodeling and motility of the plant ER rely mainly on actin [4] and to a minor extent on microtubules [5]. Although a three-way interaction between the ER, cytosolic myosin-XI, and F-actin mediates the plant ER streaming [6], the mechanisms underlying stable interaction of the ER membrane with actin are unknown. Early electron microscopy studies suggested a direct attachment of the plant ER with actin filaments [7, 8], but it is plausible that yet-unknown proteins facilitate anchoring of the ER membrane with the cytoskeleton. We demonstrate here that SYP73, a member of the plant Syp7 subgroup of SNARE proteins [9] containing actin-binding domains, is a novel ER membrane-associated actin-binding protein. We show that overexpression of SYP73 causes a striking rearrangement of the ER over actin and that, similar to mutations of myosin-XI [4, 10, 11], loss of SYP73 reduces ER streaming and affects overall ER network morphology and plant growth. We propose a model for plant ER remodeling whereby the dynamic rearrangement and streaming of the ER network depend on the propelling action of myosin-XI over actin coupled with a SYP73-mediated bridging, which dynamically anchors the ER membrane with actin filaments.

KEYWORDS:

Arabidopsis thaliana; SYP73; actin; endoplasmic reticulum; myosin

PMID:
27866894
DOI:
10.1016/j.cub.2016.10.024
[Indexed for MEDLINE]
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