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Enzyme Microb Technol. 2016 Dec;95:94-99. doi: 10.1016/j.enzmictec.2016.09.006. Epub 2016 Sep 20.

Rapid detection of viral antibodies based on multifunctional Staphylococcus aureus nanobioprobes.

Author information

1
Key Laboratory of Emerging Pathogens and Infections, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China; University of Chinese Academy of Sciences, Beijing 100039, China.
2
Key Laboratory of Emerging Pathogens and Infections, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China.
3
Key Laboratory of Emerging Pathogens and Infections, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China. Electronic address: hpwei@wh.iov.cn.

Abstract

Biosynthesis of nanoparticles inside S. aureus cells has enhanced the sensitivity of immunoassays based on the S. aureus nanoparticles. However, the current methods are limited to antigen detection by conjugating IgG antibodies on S. aureus nanoparticles. In this study, a simple way to conjugate antigens to the S. aureus nanobioparticles was developed by utilizing a cell wall binding domain (CBD) from a bacteriophage lysin PlyV12. Based on this novel design, simple agglutination tests of the IgG antibodies of Ebola virus (EBOV) nucleoprotein (NP) and Middle East Respiratory Virus (MERS) NP in rabbit sera were successfully developed by conjugating the S. aureus nanobioparticles with two fusion proteins EBOV NP- CBD and MERS NP-CBD, respectively. The conjugation was done easily by just mixing the fusion proteins with the S. aureus nanoparticles. The detection time was within 20 min without any special equipment or expertise. As far as we know, this is the first time to realize the detection of viral antibodies based on S. aureus nanoparticles.

KEYWORDS:

5-cyano-2,3-ditolyl tetrazolium chloride; Antibody detection; Staphylococcus aureus; Virus

PMID:
27866631
DOI:
10.1016/j.enzmictec.2016.09.006
[Indexed for MEDLINE]

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