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Appl Biochem Biotechnol. 2017 May;182(1):382-399. doi: 10.1007/s12010-016-2333-z. Epub 2016 Nov 18.

Isolation, Identification and High-Throughput Screening of Neutral Lipid Producing Indigenous Microalgae from South African Aquatic Habitats.

Author information

1
Discipline of Microbiology, School of Life Sciences, College of Agriculture Engineering and Sciences, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, 4000, South Africa.
2
Discipline of Microbiology, School of Life Sciences, College of Agriculture Engineering and Sciences, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, 4000, South Africa. mutanda@ukzn.ac.za.

Abstract

Exploring indigenous microalgae capable of producing significant amounts of neutral lipids through high-throughput screening is crucial for sustainable biodiesel production. In this study, 31 indigenous microalgal strains were isolated from diverse aquatic habitats in KwaZulu-Natal, South Africa. Eight superior lipid-producing strains were selected for further analysis, based on Nile red fluorescence microscopy screening. The microalgal isolates were identified to belong to the genera Chlorella, Neochloris and Chlamydomonas via morpho-taxonomic and molecular approach by 18S rRNA gene sequencing. Chlorella vulgaris PH2 had the highest specific growth rate (μ) and lowest doubling time of 0.24 day-1 and 2.89 ± 0.05 day-1, respectively. Chlorella vulgaris T4 had the highest biomass productivity of 35.71 ± 0.03 mg L-1day-1. Chlorella vulgaris PH2 had the highest lipid content of 34.28 ± 0.47 and 38 ± 9.2% (dcw) as determined by gravimetric analysis and the sulfo-phospho-vanillin (SPV) method, respectively. Chlorella vulgaris PH2 exhibited a high content of saturated fatty acids, while Chlorella sp. T4 exhibited a high total content of saturated and monounsaturated fatty acids with a low content of polyunsaturated fatty acids. The preponderance of neutral lipids suggests that Chlorella sp. T4 is a suitable candidate for biomass feedstock for biodiesel production.

KEYWORDS:

Biodiesel; Bioprospect; Chlorella sp.; Fatty acid methyl esters; GC-MS; Indigenous; Lipid profile; Microalgae

PMID:
27864781
DOI:
10.1007/s12010-016-2333-z
[Indexed for MEDLINE]

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