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Chembiochem. 2017 Jan 17;18(2):161-165. doi: 10.1002/cbic.201600359. Epub 2016 Dec 13.

Copper is a Cofactor of the Formylglycine-Generating Enzyme.

Author information

1
Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056, Basel, Switzerland.
2
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2, 8093, Zürich, Switzerland.

Abstract

Formylglycine-generating enzyme (FGE) is an O2 -utilizing oxidase that converts specific cysteine residues of client proteins to formylglycine. We show that CuI is an integral cofactor of this enzyme and binds with high affinity (KD =of 10-17  m) to a pair of active-site cysteines. These findings establish FGE as a novel type of copper enzyme.

KEYWORDS:

EPR; copper; formylglycine; oxidases; oxygen activation; protein engineering

PMID:
27862795
PMCID:
PMC5324649
DOI:
10.1002/cbic.201600359
[Indexed for MEDLINE]
Free PMC Article

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