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Plant J. 2017 Feb;89(4):671-680. doi: 10.1111/tpj.13420. Epub 2017 Feb 10.

Design of an optimal promoter involved in the heat-induced transcriptional pathway in Arabidopsis, soybean, rice and maize.

Author information

1
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, 305-8686, Japan.
2
Citrus Research Division, Institute of Fruit Tree and Tea Science, NARO, Shizuoka, Shizuoka Prefecture, 424-0292, Japan.
3
Faculty of Applied Biological Sciences and United Graduate School of Agricultural Science, Gifu University, Gifu, Gifu Prefecture, 501-1103, Japan.
4
Faculty of Education, Gifu Shotoku Gakuen University, Gifu, Gifu Prefecture, 501-6194, Japan.
5
RIKEN Bioresource Center, Koyadai 3-1-1, Tsukuba, Ibaraki, 305-0074, Japan.
6
RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
7
Research and Education Faculty, Multidisciplinary Science Cluster, Interdisciplinary Science Unit, Kochi University, Nankoku, Kochi, 783-8502, Japan.
8
Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan.

Abstract

Interactions between heat shock (HS) factors (HSFs) and heat shock response elements (HSEs) are important during the heat shock response (HSR) of flora and fauna. Moreover, plant HSFs that are involved in heat stress are also involved in abiotic stresses such as dehydration and cold as well as development, cell differentiation and proliferation. Because the specific combination of HSFs and HSEs involved in plants under heat stress remains unclear, the mechanism of their interaction has not yet been utilized in molecular breeding of plants for climate change. For the study reported herein, we compared the sequences of HS-inducible genes and their promoters in Arabidopsis, soybean, rice and maize and then designed an optimal HS-inducible promoter. Our analyses suggest that, for the four species, the abscisic acid-independent, HSE/HSF-dependent transcriptional pathway plays a major role in HS-inducible gene expression. We found that an 18-bp sequence that includes the HSE has an important role in the HSR, and that those sequences could be classified as representative of monocotyledons or dicotyledons. With the HS-inducible promoter designed based on our bioinformatic predictions, we were able to develop an optimal HS-specific inducible promoter for seedlings or single cells in roots. These findings demonstrate the utility of our HS-specific inducible promoter, which we expect will contribute to molecular breeding efforts and cell-targeted gene expression in specific plant tissues.

KEYWORDS:

Arabidopsis thaliana ; Glycine max ; Oryza sativa ; Zea mays ; cis-acting element; heat; optimal promoter; transcription

PMID:
27862521
DOI:
10.1111/tpj.13420
[Indexed for MEDLINE]
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