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New Phytol. 2017 Mar;213(4):1726-1739. doi: 10.1111/nph.14301. Epub 2016 Nov 15.

Respiration climacteric in tomato fruits elucidated by constraint-based modelling.

Author information

1
UMR 1332 Biologie du Fruit et Pathologie, INRA, Villenave d'Ornon, F-33883, France.
2
Université de Bordeaux, 146 rue Léo-Saignat, Bordeaux Cedex, F-33076, France.
3
ENSTBB-Institut Polytechnique de Bordeaux, Institut de Mathématiques de Bordeaux, 351 Cours de la Liberation, Talence, 33400, France.
4
UR 1115 Plantes et Systèmes de culture Horticoles, INRA, Avignon Cedex 9, F84914, France.
5
UR 1268 Biopolymères, Interactions, Assemblages, INRA, Nantes, F-44316, France.
6
IBGC-CNRS, 1 rue Camille Saint-Saëns, Bordeaux Cedex, F-33077, France.

Abstract

Tomato is a model organism to study the development of fleshy fruit including ripening initiation. Unfortunately, few studies deal with the brief phase of accelerated ripening associated with the respiration climacteric because of practical problems involved in measuring fruit respiration. Because constraint-based modelling allows predicting accurate metabolic fluxes, we investigated the respiration and energy dissipation of fruit pericarp at the breaker stage using a detailed stoichiometric model of the respiratory pathway, including alternative oxidase and uncoupling proteins. Assuming steady-state, a metabolic dataset was transformed into constraints to solve the model on a daily basis throughout tomato fruit development. We detected a peak of CO2 released and an excess of energy dissipated at 40 d post anthesis (DPA) just before the onset of ripening coinciding with the respiration climacteric. We demonstrated the unbalanced carbon allocation with the sharp slowdown of accumulation (for syntheses and storage) and the beginning of the degradation of starch and cell wall polysaccharides. Experiments with fruits harvested from plants cultivated under stress conditions confirmed the concept. We conclude that modelling with an accurate metabolic dataset is an efficient tool to bypass the difficulty of measuring fruit respiration and to elucidate the underlying mechanisms of ripening.

KEYWORDS:

carbon allocation; central metabolism; constraint-based model; environmental stress; flux balance analysis (FBA); metabolic flux modelling; respiration climacteric; tomato fruit development

PMID:
27861943
PMCID:
PMC6079640
DOI:
10.1111/nph.14301
[Indexed for MEDLINE]
Free PMC Article

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