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Adv Healthc Mater. 2017 Jan;6(2). doi: 10.1002/adhm.201600804. Epub 2016 Nov 8.

Improved Anticancer Photothermal Therapy Using the Bystander Effect Enhanced by Antiarrhythmic Peptide Conjugated Dopamine-Modified Reduced Graphene Oxide Nanocomposite.

Author information

1
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
2
Department of Food and Beverage Management, Taipei College of Maritime Technology, Taipei, 11174, Taiwan.
3
Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, 20224, Taiwan.
4
The National Key Laboratory of Shock Wave and Detonation Physics, Institute of Fluid Physics, CAEP, Mianyang, 621900, China.
5
Research Center for Environmental Changes, Academia Sinica, Taipei, 11529, Taiwan.
6
Department of Food Science, National Taiwan Ocean University, Keelung, 20224, Taiwan.
7
Department of Biotechnology, National Formosa University, Yunlin, 63208, Taiwan.

Abstract

Despite tremendous efforts toward developing novel near-infrared (NIR)-absorbing nanomaterials, improvement in therapeutic efficiency remains a formidable challenge in photothermal cancer therapy. This study aims to synthesize a specific peptide conjugated polydopamine-modified reduced graphene oxide (pDA/rGO) nanocomposite that promotes the bystander effect to facilitate cancer treatment using NIR-activated photothermal therapy. To prepare a nanoplatform capable of promoting the bystander effect in cancer cells, we immobilized antiarrhythmic peptide 10 (AAP10) on the surface of dopamine-modified rGO (AAP10-pDA/rGO). Our AAP10-pDA/rGO could promote the bystander effect by increasing the expression of connexin 43 protein in MCF-7 breast-cancer cells. Because of its tremendous ability to absorb NIR absorption, AAP10-pDA/rGO offers a high photothermal effect under NIR irradiation. This leads to a massive death of MCF-7 cells via the bystander effect. Using tumor-bearing mice as the model, it is found that NIR radiation effectively ablates breast tumor in the presence of AAP10-pDA/rGO and inhibits tumor growth by ≈100%. Therefore, this research integrates the bystander and photothermal effects into a single nanoplatform in order to facilitate an efficient photothermal therapy. Furthermore, our AAP10-pDA/rGO, which exhibits both hyperthermia and the bystander effect, can prevent breast-cancer recurrence and, therefore, has great potential for future clinical and research applications.

KEYWORDS:

breast-cancer cells; bystander effect; near-infrared; phototherapy; reduced graphene oxide

PMID:
27860462
DOI:
10.1002/adhm.201600804
[Indexed for MEDLINE]

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