Send to

Choose Destination
Anat Rec (Hoboken). 2017 Jun;300(6):1114-1122. doi: 10.1002/ar.23517. Epub 2017 Feb 12.

Ionized calcium-binding adaptor molecule 1 positive macrophages and HO-1 up-regulation in intestinal muscularis resident macrophages.

Author information

Department of Cellular and Molecular Medicine, University of Copenhagen, Denmark.
Department of Medicine Faculty of Health Sciences, Farncombe Family Digestive Health Research Institute McMaster University, Canada.
Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark.
Department of Oral Medicine Faculty of Health Sciences, University of Copenhagen, Denmark.


Small intestinal muscularis externa macrophages have been associated with interstitial cells of Cajal. They have been proposed to play various roles in motility disorders and to take part in a microbiota-driven regulation of gastrointestinal motility. Our objective was to understand the reaction of resident macrophages of the musculature to a pro-inflammatory stimulator, lipopolysaccharide (LPS). Mice were injected with LPS or saline and sacrificed after 6 hr. Whole mounts were stained with antibodies toward CD169, ionized calcium-binding adaptor molecule 1 (iba1) (microglial/macrophage marker) and heme oxygenase-1 (HO-1). Cell densities were measured using unbiased stereology.


iba1pos cells showed an overall higher density than CD169pos and HO-1pos cells. Most HO-1pos and iba1pos cells were positive for CD 169 in serosa and at Auerbach's plexus (AP). At the deep muscular plexus, mainly iba1pos cells were present, and were mostly CD169neg ; a few HO-1pos cells were present.


A new subset of resident macrophages in the intestinal muscularis externa was discovered, identified as iba1pos CD169neg . HO-1 is constitutively present in most macrophages in serosa and at AP, suggesting a M2 phenotype. LPS-treatment results in an up-regulation of HO-1pos /CD169neg cells in serosa and at AP. Anat Rec, 300:1114-1122, 2017. © 2016 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.


HO-1; LPS; iba1; immunohistochemistry; intestine; macrophages

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center