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Appl Microbiol Biotechnol. 2017 Feb;101(4):1605-1614. doi: 10.1007/s00253-016-7953-2. Epub 2016 Nov 17.

Lactobacillus plantarum HAC01 regulates gut microbiota and adipose tissue accumulation in a diet-induced obesity murine model.

Author information

1
Advanced Green Energy and Environment Institute (AGEE), Handong Global University, 558 Handong-Ro, 3, Heunghae-eup, Buk-gu, Pohang, Gyungbuk, 37673, South Korea.
2
R&D Center, Pohang, Gyungbuk, 37668, South Korea.
3
Department of Life Science, Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang, Gyungbuk, 37673, South Korea.
4
AtoGen Co. Ltd., 11-8 Techno 1-ro, Yuseong-gu, Daejeon, 34015, South Korea.
5
School of Life Sciences, Handong Global University, Pohang, Gyungbuk, 791-708, South Korea.
6
Advanced Green Energy and Environment Institute (AGEE), Handong Global University, 558 Handong-Ro, 3, Heunghae-eup, Buk-gu, Pohang, Gyungbuk, 37673, South Korea. wilhelm@woodapple.net.

Abstract

The functional features of Lactobacillus plantarum HAC01 (HAC01), isolated from fermented Korean kimchi, were studied with regard to the fat mass, immunometabolic biomarkers and dysbiosis in a diet-induced obesity (DIO) murine model. L. rhamnosus GG (LGG) served as reference strain and a PBS-treated group as control. The administration of L. plantarum HAC01 resulted in reduction of the mesenteric adipose depot, the conjunctive tissue closely associated with the gastrointestinal tract, where lipid oxidative gene expression was upregulated compared to the control group. Metagenome analysis of intestinal microbiota showed that both strains HAC01 and LGG influenced specific bacterial families such as the Lachnospiraceae and Ruminococcaceae rather than the phyla Firmicutes and Bacteroidetes as a whole. The relative abundance of the Lachnospiraceae (phylum Firmicutes) was significantly higher in both LAB-treated groups than in the control. Comparing the impact of the two Lactobacillus strains on microbial composition in the gut also suggests strain-specific effects. The study emphasises the need for deeper studies into functional specificity of a probiotic organism at the strain level. Alleviation of obesity-associated dysbiosis by modulation of the gut microbiota appears to be associated with "indicator" bacterial taxa such as the family Lachnospiraceae. This may provide further insight into mechanisms basic to the mode of probiotic action against obesity and associated dysbiosis.

KEYWORDS:

Diet-induced obesity; Dysbiosis; Gut microbiota; Lactobacillus plantarum; Lactobacillus rhamnosus GG; Mesenteric adipose tissue

PMID:
27858139
DOI:
10.1007/s00253-016-7953-2
[Indexed for MEDLINE]

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