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G3 (Bethesda). 2017 Jan 5;7(1):129-142. doi: 10.1534/g3.116.034298.

The Neurospora Transcription Factor ADV-1 Transduces Light Signals and Temporal Information to Control Rhythmic Expression of Genes Involved in Cell Fusion.

Author information

1
Department of Biology, Texas A&M University, College Station, Texas 77843.
2
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331.
3
Bioinformatics Program, Boston University, Massachusetts 02215.
4
Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755.
5
Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York 12180.
6
National Emerging Infectious Diseases Laboratories, Boston University, Massachusetts 02118.
7
Department of Microbiology, Boston University, Massachusetts 02215.
8
Department of Biomedical Engineering, Boston University, Massachusetts 02215.
9
Department of Biology, Texas A&M University, College Station, Texas 77843 dpedersen@bio.tamu.edu.

Abstract

Light and the circadian clock have a profound effect on the biology of organisms through the regulation of large sets of genes. Toward understanding how light and the circadian clock regulate gene expression, we used genome-wide approaches to identify the direct and indirect targets of the light-responsive and clock-controlled transcription factor ADV-1 in Neurospora crassa A large proportion of ADV-1 targets were found to be light- and/or clock-controlled, and enriched for genes involved in development, metabolism, cell growth, and cell fusion. We show that ADV-1 is necessary for transducing light and/or temporal information to its immediate downstream targets, including controlling rhythms in genes critical to somatic cell fusion. However, while ADV-1 targets are altered in predictable ways in Δadv-1 cells in response to light, this is not always the case for rhythmic target gene expression. These data suggest that a complex regulatory network downstream of ADV-1 functions to generate distinct temporal dynamics of target gene expression relative to the central clock mechanism.

KEYWORDS:

circadian clock; development; phototransduction; regulatory network

PMID:
27856696
PMCID:
PMC5217103
DOI:
10.1534/g3.116.034298
[Indexed for MEDLINE]
Free PMC Article

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