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Microb Pathog. 2016 Dec;101:119-125. doi: 10.1016/j.micpath.2016.11.008. Epub 2016 Nov 14.

Antimicrobial, antitrypanosomal and antibiofilm activity of Equisetum hyemale.

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Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
Department of Clinical and Toxicological Analysis, Mycobacteriology Laboratory, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
Laboratory of Microbiological Research, Centro Universitário Franciscano (UNIFRA), Santa Maria, Brazil.
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Laboratory of Pharmacotechnique, Universidade Regional Integrada, URI-Santiago, Santiago, Brazil.
Oral Microbiology Research Laboratory, Microbiology and Parasitology Department, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.


This study evaluates, for the first time, the antibiofilm, antimicrobial and antiparasitic potential of crude extract and fractions of stems of Equisetum hyemale against several infectious agents (bacteria, fungi, Mycobacterium and Trypanosomes) by broth microdilution technique and investigates the phenolic composition of the plant by high performance liquid chromatography. The crude extract and fractions showed antimicrobial activity, as they were capable of inhibiting the growth of bacteria in minimal inhibitory concentrations (MICs) ranging from 52.4 mg/mL to 3.27 mg/mL. For Candida species, the MICs ranged from 52.4 mg/mL to 6.5 mg/mL, and for Mycobacterium species from 2.5 mg/mL to 0.625 mg/mL. The dichloromethane fraction was able to reduce 83% of Pseudomonas aeruginosa biofilm formation and 51% of Candida albicans biofilms. The n-butanol fraction presents an important protozoal effect, reducing 100% of Trypanosoma evansi trypomastigotes after 9 h of exposure. The HPLC analysis revealed that the major substances are rosmarinic acid in dichloromethane fraction (7.38 ± 0.08 mg/g FS) and chlorogenic acid in ethyl acetate fraction (8.4 ± 0.26 mg/g FS). The crude extract and fractions of E. hyemale can be both useful and effective agents as a sustainable alternative for the treatment and prevention of several infectious agents.


Candida; Natural products; Phytochemical characterization; Pseudomonas aeruginosa PA01; Trypanosoma evansi

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