Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection

Paul M Lizardi; Qin Yan; Narendra Wajapeyee

doi:10.1101/pdb.prot094847

Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection

Cold Spring Harb Protoc. 2017 Dec 1;2017(12):pdb.prot094847. doi: 10.1101/pdb.prot094847.

Authors

Paul M Lizardi, Qin Yan, Narendra Wajapeyee

PMID: 27852838
DOI: 10.1101/pdb.prot094847

Abstract

Methylation-specific polymerase chain reaction (MS-PCR) is a more rapid way to detect changes in DNA methylation than is bisulfite sequencing. In addition, by incorporating some basic automation, samples can be prepared and analyzed in a 96-well plate format. The method can be used either quantitatively (qRT-PCR-based MethyLight) or qualitatively (using agarose gels) to detect changes in DNA methylation; both are described in this protocol.

MeSH terms

DNA / chemistry*
DNA / genetics*
DNA Methylation*
Polymerase Chain Reaction / methods*

Substances

DNA