Format

Send to

Choose Destination
J Exp Med. 2016 Dec 12;213(13):2913-2929. Epub 2016 Nov 16.

Dampened antiviral immunity to intravaginal exposure to RNA viral pathogens allows enhanced viral replication.

Author information

1
Virology and Immunology, Gladstone Institutes, San Francisco, CA 94158.
2
Department of Medicine, University of California, San Francisco, San Francisco, CA 94143.
3
Virology and Immunology, Gladstone Institutes, San Francisco, CA 94158 shomyseh.sanjabi@gladstone.ucsf.edu.
4
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143.

Abstract

Understanding the host immune response to vaginal exposure to RNA viruses is required to combat sexual transmission of this class of pathogens. In this study, using lymphocytic choriomeningitis virus (LCMV) and Zika virus (ZIKV) in wild-type mice, we show that these viruses replicate in the vaginal mucosa with minimal induction of antiviral interferon and inflammatory response, causing dampened innate-mediated control of viral replication and a failure to mature local antigen-presenting cells (APCs). Enhancement of innate-mediated inflammation in the vaginal mucosa rescues this phenotype and completely inhibits ZIKV replication. To gain a better understanding of how this dampened innate immune activation in the lower female reproductive tract may also affect adaptive immunity, we modeled CD8 T cell responses using vaginal LCMV infection. We show that the lack of APC maturation in the vaginal mucosa leads to a delay in CD8 T cell activation in the draining lymph node and hinders the timely appearance of effector CD8 T cells in vaginal mucosa, thus further delaying viral control in this tissue. Our study demonstrates that vaginal tissue is exceptionally vulnerable to infection by RNA viruses and provides a conceptual framework for the male to female sexual transmission observed during ZIKV infection.

PMID:
27852793
PMCID:
PMC5154948
DOI:
10.1084/jem.20161289
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center