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Pathol Oncol Res. 2017 Jul;23(3):551-564. doi: 10.1007/s12253-016-0144-8. Epub 2016 Nov 14.

Molecular Profiling of Thymoma and Thymic Carcinoma: Genetic Differences and Potential Novel Therapeutic Targets.

Author information

1
Department of Pathology, Medical University Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
2
Center for Integrative Bioinformatics Vienna, Max F. Perutz Laboratories, University of Vienna and Medical University Vienna, Vienna, Austria.
3
Department of Thoracic Surgery, Medical University Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
4
Center for Medical Statistics, Informatics, and Intelligent Systems, Section for Biosimulation and Bioinformatic, Medical University Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
5
Department of Pathology, Medical University Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria. leonhard.muellauer@meduniwien.ac.at.

Abstract

Thymoma and thymic carcinoma are thymic epithelial tumors (TETs). We performed a molecular profiling to investigate the pathogenesis of TETs and identify novel targets for therapy. We analyzed 37 thymomas (18 type A, 19 type B3) and 35 thymic carcinomas. The sequencing of 50 genes detected nonsynonymous mutations in 16 carcinomas affecting ALK, ATM, CDKN2A, ERBB4, FGFR3, KIT, NRAS and TP53. Only two B3 thymomas had a mutation in noncoding regions of the SMARCB1 and STK11 gene respectively. Three type A thymomas harbored a nonsynonymous HRAS mutation. Fluorescence in situ hybridization detected in 38 % of carcinomas a CDKN2A, in 32 % a TP53 and in 8 % an ATM gene deletion, whereas only one B3 thymoma exhibited a CDKNA deletion, and none of the type A thymomas showed a gene loss. Sequencing of the total miRNA pool of 5 type A thymomas and 5 thymic carcinomas identified the C19MC miRNA cluster as highly expressed in type A thymomas, but completely silenced in thymic carcinomas. Furthermore, the miRNA cluster C14MC was downregulated in thymic carcinomas. Among non-clustered miRNAs, the upregulation of miR-21, miR-9-3 and miR-375 and the downregulation of miR-34b, miR-34c, miR-130a and miR-195 in thymic carcinomas were most significant. The expression of ALK, HER2, HER3, MET, phospho-mTOR, p16INK4A, PDGFRA, PDGFRB, PD-L1, PTEN and ROS1 was investigated by immunohistochemistry. PDGFRA was increased in thymic carcinomas and PD-L1 in B3 thymomas and thymic carcinomas. In summary, our results reveal genetic differences between thymomas and thymic carcinomas and suggest potential novel targets for therapy.

KEYWORDS:

Immunohistochemistry; Mutation; Thymic carcinoma; Thymoma; miRNA

PMID:
27844328
PMCID:
PMC5487866
DOI:
10.1007/s12253-016-0144-8
[Indexed for MEDLINE]
Free PMC Article

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