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Mol Cell Biochem. 2017 Jan;425(1-2):113-123. doi: 10.1007/s11010-016-2867-y. Epub 2016 Nov 14.

Effect of puerarin on transcriptome of astrocyte during oxygen-glucose deprivation/reoxygenation injury.

Author information

1
Southwest University, Rongchang campus, Rongchang, Chongqing, 402460, China. Shuyongwei013@163.com.
2
Pharmaceutical Sciences College & Chinese Medicine College, Southwest University, Chongqing Engineering Research Center for Pharmacodynamics Evaluation, Chongqing, 400716, China.
3
Pharmaceutical Sciences College & Chinese Medicine College, Southwest University, Chongqing Engineering Research Center for Pharmacodynamics Evaluation, Chongqing, 400716, China. xuxiaoyu@swu.edu.cn.

Abstract

Stroke is a serious disease with complex pathomechanism and limited therapeutic effect in clinic. Our previous research has found obvious therapeutic effect of Puerarin (Pur) on stroke injury of rat. The aim of this study is to investigate the transcriptome changes of oxygen-glucose deprivation/reoxygenation (OGD/R)-injured astrocytes before and after the intervention of Pur. Cells activity and apoptosis detection indicated that the activity of OGD/R-injured astrocytes was improved, and the apoptosis was ameliorated by Pur. Affymetrix GeneChip Rat Genome 230 2.0 Array assays indicated that after intervention of Pur, mRNA expressions of 31 genes were up-regulated and 40 genes were down-regulated in OGD group, whereas mRNA expression of 36 genes were up-regulated, and 88 genes were down-regulated in OGD/R group. Pathway analysis indicated that the olfactory transduction pathway and the JAK (janus kinase) 2/STAT (signal transducer and activator of transcription) three pathways were down-regulated by Pur during OGD/R injury of astrocytes. These data indicated that Pur regulates transcriptome and expresses protective effect on astrocytes during OGD/R injury, and may be a potential therapeutic agent for the treatment of stroke.

KEYWORDS:

Astrocytes; IL-6/JAK2/STAT3 pathway; OGD/R; Puerarin; Transcriptome

PMID:
27844252
DOI:
10.1007/s11010-016-2867-y
[Indexed for MEDLINE]

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