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J Vis Exp. 2016 Oct 31;(116). doi: 10.3791/54666.

Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes.

Author information

1
Waksman Institute, Rutgers University.
2
Waksman Institute, Rutgers University; Department of Genetics, Rutgers University; mckim@rci.rutgers.edu.

Abstract

Chromosome segregation in human oocytes is error prone, resulting in aneuploidy, which is the leading genetic cause of miscarriage and birth defects. The study of chromosome behavior in oocytes from model organisms holds much promise to uncover the molecular basis of the susceptibility of human oocytes to aneuploidy. Drosophila melanogaster is amenable to genetic manipulation, with over 100 years of research, community, and technique development. Visualizing chromosome behavior and spindle assembly in Drosophila oocytes has particular challenges, however, due primarily to the presence of membranes surrounding the oocyte that are impenetrable to antibodies. We describe here protocols for the collection, preparation, and imaging of meiosis I spindle assembly and chromosome behavior in Drosophila oocytes, which allow the molecular dissection of chromosome segregation in this important model organism.

PMID:
27842371
PMCID:
PMC5226183
DOI:
10.3791/54666
[Indexed for MEDLINE]
Free PMC Article

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