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  • PMID: 27841783 was deleted because it is a duplicate of PMID: 28060071
Cornea. 2017 Feb;36(2):222-228. doi: 10.1097/ICO.0000000000001076.

Corneal Lenticule Allotransplantation After Femtosecond Laser Small Incision Lenticule Extraction in Rabbits.

Author information

1
Key Laboratory of Myopia, Ministry of Health, Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.

Abstract

PURPOSE:

To investigate the feasibility of allotransplanting extracted lenticules after femtosecond laser-assisted small incision lenticule extraction (SMILE) in rabbits and the subsequent healing process.

METHODS:

Fourteen New Zealand white rabbits were divided evenly into 2 groups. The rabbits in group A received SMILE procedures with a -6.00 D correction. The lenticules from group A were immediately inserted into a femtosecond laser-created corneal stromal pocket in group B. After surgery, the anterior segment was assessed in vivo by slit-lamp microscopy, corneal topography, optical coherence tomography, and confocal microscopy. All eyes were enucleated for hematoxylin-eosin staining and transmission electron microscopy after the animals were killed.

RESULTS:

At postoperative day 1, there was moderate corneal edema in the implanted lenticule stroma. At 6 months, the lenticules were integrated with the surrounding tissue, and the boundary could not be identified through slit-lamp microscopy; regenerated branches of the corneal nerves were thicker than at postoperative month 1 as observed through confocal microscopy. The central corneal thickness increased by 58.75 ± 21.58 μm. The lenticules were gradually integrated with the surrounding tissue, and their density was similar to the adjacent tissue according to optical coherence tomography; however, a clear boundary between the lenticule and surrounding tissue was detectable using light microscopy and transmission electron microscopy, revealing disordered fibers and decreased keratocytes in implanted lenticules.

CONCLUSIONS:

In this model it is feasible and safe to allotransplant extracted corneal lenticules after SMILE. Healing of implanted lenticules after SMILE is stable at postoperative 6 months, but collagen fiber rearrangement requires further investigation.

PMID:
28060071
DOI:
10.1097/ICO.0000000000001076
[Indexed for MEDLINE]

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