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Cell Stem Cell. 2017 Jan 5;20(1):56-69. doi: 10.1016/j.stem.2016.09.010. Epub 2016 Nov 10.

Myogenic Progenitor Cells Control Extracellular Matrix Production by Fibroblasts during Skeletal Muscle Hypertrophy.

Author information

1
Department of Nutrition and Metabolism, University of Texas Medical Branch, Galveston, TX 77555, USA.
2
Department of Physiology, University of Kentucky, Lexington, KY 40536, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY 40536, USA.
3
Center for Muscle Biology, University of Kentucky, Lexington, KY 40536, USA; College of Health Sciences, University of Kentucky, Lexington, KY 40536, USA.
4
Department of Physiology, University of Kentucky, Lexington, KY 40536, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY 40536, USA. Electronic address: jjmcca2@uky.edu.
5
Department of Physiology, University of Kentucky, Lexington, KY 40536, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY 40536, USA; College of Health Sciences, University of Kentucky, Lexington, KY 40536, USA. Electronic address: cpete4@uky.edu.

Abstract

Satellite cells, the predominant stem cell population in adult skeletal muscle, are activated in response to hypertrophic stimuli and give rise to myogenic progenitor cells (MPCs) within the extracellular matrix (ECM) that surrounds myofibers. This ECM is composed largely of collagens secreted by interstitial fibrogenic cells, which influence satellite cell activity and muscle repair during hypertrophy and aging. Here we show that MPCs interact with interstitial fibrogenic cells to ensure proper ECM deposition and optimal muscle remodeling in response to hypertrophic stimuli. MPC-dependent ECM remodeling during the first week of a growth stimulus is sufficient to ensure long-term myofiber hypertrophy. MPCs secrete exosomes containing miR-206, which represses Rrbp1, a master regulator of collagen biosynthesis, in fibrogenic cells to prevent excessive ECM deposition. These findings provide insights into how skeletal stem and progenitor cells interact with other cell types to actively regulate their extracellular environments for tissue maintenance and adaptation.

KEYWORDS:

Pax7; collagen; exosomes; extracellular matrix; fibrosis; hypertrophy; microRNA; muscle; muscle progenitor cells; satellite cells

PMID:
27840022
PMCID:
PMC5218963
DOI:
10.1016/j.stem.2016.09.010
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors declare no conflicts of interest.

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