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J Microbiol Methods. 2017 Jan;132:1-3. doi: 10.1016/j.mimet.2016.11.004. Epub 2016 Nov 9.

Application of a dual target PCR-high resolution melting (HRM) method for rapid nontuberculous mycobacteria identification.

Author information

1
Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region.
2
Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region.
3
Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: kyyuen@hkucc.hku.hk.

Abstract

Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.

KEYWORDS:

High-resolution melting; Nontuberculous mycobacteria

PMID:
27838538
DOI:
10.1016/j.mimet.2016.11.004
[Indexed for MEDLINE]

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