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Sensors (Basel). 2016 Nov 8;16(11). pii: E1871.

A Portable Smart-Phone Readout Device for the Detection of Mercury Contamination Based on an Aptamer-Assay Nanosensor.

Author information

1
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China. xkevent@stu2014.jnu.edu.cn.
2
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China. cf412xm@stu2015.jnu.edu.cn.
3
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China. 1423221001@stu2014.jnu.edu.cn.
4
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China. Shiting@stu2014.jnu.edu.cn.
5
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China. alan@stu2015.jnu.edu.cn.
6
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China. alexis@stu2015.jnu.edu.cn.
7
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China. tyjaq7926@163.com.
8
Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, China. tyjaq7926@163.com.
9
Institute of Biotranslational Medicine, Jinan University, Guangzhou 510632, China. tyjaq7926@163.com.

Abstract

The detection of environmental mercury (Hg) contamination requires complex and expensive instruments and professional technicians. We present a simple, sensitive, and portable Hg2+ detection system based on a smartphone and colorimetric aptamer nanosensor. A smartphone equipped with a light meter app was used to detect, record, and process signals from a smartphone-based microwell reader (MR S-phone), which is composed of a simple light source and a miniaturized assay platform. The colorimetric readout of the aptamer nanosensor is based on a specific interaction between the selected aptamer and Hg2+, which leads to a color change in the reaction solution due to an aggregation of gold nanoparticles (AuNPs). The MR S-phone-based AuNPs-aptamer colorimetric sensor system could reliably detect Hg2+ in both tap water and Pearl River water samples and produced a linear colorimetric readout of Hg2+ concentration in the range of 1 ng/mL-32 ng/mL with a correlation of 0.991, and a limit of detection (LOD) of 0.28 ng/mL for Hg2+. The detection could be quickly completed in only 20 min. Our novel mercury detection assay is simple, rapid, and sensitive, and it provides new strategies for the on-site detection of mercury contamination in any environment.

KEYWORDS:

colorimetric aptamer nanosensor; gold nanoparticle; mercury ions; microwell reader; smartphone

PMID:
27834794
PMCID:
PMC5134530
DOI:
10.3390/s16111871
[Indexed for MEDLINE]
Free PMC Article

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