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Indian J Med Res. 2016 Jul;144(1):32-37. doi: 10.4103/0971-5916.193280.

Association of CTG repeat polymorphism in carnosine dipeptidase 1 (CNDP1) gene with diabetic nephropathy in north Indians.

Author information

1
Department of Nephrology, Post Graduate Institute of Medical Education & Research, Chandigarh, India.
2
Department of Endocrinology, Post Graduate Institute of Medical Education & Research, Chandigarh, India.
3
Department of Nephrology, Post Graduate Institute of Medical Education & Research, Chandigarh; George Institute for Global Health, New Delhi, India.

Abstract

BACKGROUND & OBJECTIVES:

CNDP1 gene, present on chromosome 18q22.3-23, encodes carnosinase, the rate-limiting enzyme in hydrolysis of carnosine to ß-alanine and L-histidine. Linkage of CTG trinucleotide (leucine) repeat polymorphism in CNDP1 gene with diabetic nephropathy has been observed in several populations. However, this association is conflicting and population-dependent. We investigated this association in type 2 diabetes mellitus (T2DM) patients with and without nephropathy in north India.

METHODS:

A total of 564 individuals [199 T2DM without nephropathy (DM), 185 T2DM with nephropathy (DN) and 180 healthy individuals (HC)] were enrolled. CNDP1 CTG repeat analysis was done by direct sequencing of a 377 base pair fragment in exon 2.

RESULTS:

The most frequent leucine (L) repeats were 5L-5L, 6L-5L and 6L-6L. 5L-5L genotype frequency was reduced in DN (24.3%) as compared to DM (34.7%, P=0.035) and HC (38.4%, P=0.005). Similarly, 5L allele frequency was lower in DN (46.8%) as compared to DM (57.3%, P=0.004) and HC (60.5%, P<0.001). The genotype and allelic frequencies were similar in DM and HC groups. No gender specific difference was observed in the genotype or allelic frequencies between groups.

INTERPRETATION & CONCLUSIONS:

Compared to healthy individuals and those with diabetes but no kidney disease, patients with diabetic nephropathy exhibited lower frequencies of 5L-5L genotype and 5L allele of CNDP1 gene, suggesting that this allele might confer protection against development of kidney disease in this population.

PMID:
27834323
PMCID:
PMC5116895
DOI:
10.4103/0971-5916.193280
[Indexed for MEDLINE]
Free PMC Article

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