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Cell Host Microbe. 2016 Nov 9;20(5):618-630. doi: 10.1016/j.chom.2016.10.015.

Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes.

Author information

1
Malaria Biology and Genetics Unit, Pasteur Institute, Paris 75015, France; Department of Parasitology, University of São Paulo-USP, São Paulo 05508-000, SP, Brazil. Electronic address: danielbargieri@gmail.com.
2
Malaria Biology and Genetics Unit, Pasteur Institute, Paris 75015, France.
3
Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
4
Malaria Biology and Genetics Unit, Pasteur Institute, Paris 75015, France; Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA.
5
Division of Cellular and Applied Infection Biology, Institute of Zoology, RWTH Aachen University, Aachen 52074, Germany.
6
Inserm U1016, CNRS UMR 8104, Université Paris Descartes, Institut Cochin, Paris 75014, France.
7
Malaria Parasite Biology and Vaccines Unit, Pasteur Institute, Paris 75015, France.
8
The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, VIC, Australia.
9
Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Ehime 790-8577, Japan.
10
The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, VIC, Australia; Department of Medical Biology, University of Melbourne, Parkville 3052, VIC, Australia.
11
Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Rome 00161, Italy.

Abstract

Surface-associated TRAP (thrombospondin-related anonymous protein) family proteins are conserved across the phylum of apicomplexan parasites. TRAP proteins are thought to play an integral role in parasite motility and cell invasion by linking the extracellular environment with the parasite submembrane actomyosin motor. Blood stage forms of the malaria parasite Plasmodium express a TRAP family protein called merozoite-TRAP (MTRAP) that has been implicated in erythrocyte invasion. Using MTRAP-deficient mutants of the rodent-infecting P. berghei and human-infecting P. falciparum parasites, we show that MTRAP is dispensable for erythrocyte invasion. Instead, MTRAP is essential for gamete egress from erythrocytes, where it is necessary for the disruption of the gamete-containing parasitophorous vacuole membrane, and thus for parasite transmission to mosquitoes. This indicates that motor-binding TRAP family members function not just in parasite motility and cell invasion but also in membrane disruption and cell egress.

PMID:
27832590
PMCID:
PMC5104695
DOI:
10.1016/j.chom.2016.10.015
[Indexed for MEDLINE]
Free PMC Article

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