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Nat Commun. 2016 Nov 10;7:13271. doi: 10.1038/ncomms13271.

DciA is an ancestral replicative helicase operator essential for bacterial replication initiation.

Author information

1
Universite de Versailles Saint-Quentin, 45 Avenue des Etats-Unis, Versailles 78000, France.
2
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette 91198, France.

Abstract

Delivery of the replicative helicase onto DNA is an essential step in the initiation of replication. In bacteria, DnaC (in Escherichia coli) and DnaI (in Bacillus subtilis) are representative of the two known mechanisms that assist the replicative helicase at this stage. Here, we establish that these two strategies cannot be regarded as prototypical of the bacterial domain since dnaC and dnaI (dna[CI]) are present in only a few bacterial phyla. We show that dna[CI] was domesticated at least seven times through evolution in bacteria and at the expense of one gene, which we rename dciA (dna[CI] antecedent), suggesting that DciA and Dna[CI] share a common function. We validate this hypothesis by establishing in Pseudomonas aeruginosa that DciA possesses the attributes of the replicative helicase-operating proteins associated with replication initiation.

PMID:
27830752
PMCID:
PMC5109545
DOI:
10.1038/ncomms13271
[Indexed for MEDLINE]
Free PMC Article

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