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Methods Mol Biol. 2017;1506:193-200.

Minimally Invasive Liver Preconditioning for Hepatocyte Transplantation in Rats.

Gaillard M1,2,3, Dagher I4,5,6.

Author information

1
INSERM U1193, Paul Brousse Hospital, Villejuif, F-91807, France.
2
Department of Digestive Minimally Invasive Surgery, Antoine Béclère Hospital, AP-HP, Clamart, F-92140, France.
3
Faculté de Médecine Paris-Sud, Paris-Saclay University, Orsay, F-91405, France.
4
INSERM U1193, Paul Brousse Hospital, Villejuif, F-91807, France. ibrahim.dagher@abc.aphp.fr.
5
Department of Digestive Minimally Invasive Surgery, Antoine Béclère Hospital, AP-HP, Clamart, F-92140, France. ibrahim.dagher@abc.aphp.fr.
6
Faculté de Médecine Paris-Sud, Paris-Saclay University, Orsay, F-91405, France. ibrahim.dagher@abc.aphp.fr.

Abstract

In the context of cell transplantation in the liver parenchyma, preconditioning is essential to enhance cell engraftment and liver repopulation. The authors have developed a minimally invasive technique of temporary portal embolization using an absorbable material, called reversible portal vein embolization. We hereby describe the method for isolating hepatocytes from a donor rat before transplanting hepatocytes after reversible portal vein embolization in the recipient.

KEYWORDS:

Hepatocyte isolation; Hepatocyte transplantation; Liver perfusion; Liver preconditioning; Portal vein embolization

PMID:
27830554
DOI:
10.1007/978-1-4939-6506-9_13
[Indexed for MEDLINE]

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