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Arch Microbiol. 2017 Apr;199(3):465-474. doi: 10.1007/s00203-016-1314-5. Epub 2016 Nov 9.

Inhibition of the multidrug efflux pump LmrS from Staphylococcus aureus by cumin spice Cuminum cyminum.

Author information

1
Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA.
2
Department of Biological Sciences, College of Arts and Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX, 76203, USA.
3
Bioinformatics Division, Osmania University, Hyderabad, Telangana, 500007, India.
4
Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA. Manuel.Varela@enmu.edu.

Abstract

Staphylococcus aureus is a serious causative agent of infectious disease. Multidrug-resistant strains like methicillin-resistant S. aureus compromise treatment efficacy, causing significant morbidity and mortality. Active efflux represents a major antimicrobial resistance mechanism. The proton-driven multidrug efflux pump, LmrS, actively exports structurally distinct antimicrobials. To circumvent resistance and restore clinical efficacy of antibiotics, efflux pump inhibitors are necessary, and natural edible spices like cumin are potential candidates. The mode of cumin antibacterial action and underlying mechanisms behind drug resistance inhibition, however, are unclear. We tested the hypothesis that cumin inhibits LmrS drug transport. We found that cumin inhibited bacterial growth and LmrS ethidium transport in a dosage-dependent manner. We demonstrate that cumin is antibacterial toward a multidrug-resistant host and that resistance modulation involves multidrug efflux inhibition.

KEYWORDS:

Cumin; Dietary spice; Efflux; Efflux pump inhibitor; LmrS; MRSA; Multidrug resistance; Natural antimicrobials; Spices; Staphylococcus aureus

PMID:
27830269
DOI:
10.1007/s00203-016-1314-5
[Indexed for MEDLINE]

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