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BMC Biotechnol. 2016 Nov 9;16(1):78.

Repair of cartilage defects in osteoarthritis rats with induced pluripotent stem cell derived chondrocytes.

Author information

1
Shenzhen Key Laboratory for Anti-ageing and Regenerative Medicine, Health Science Center, Shenzhen University, Shenzhen, 518060, China. yanxiazhu@szu.edu.cn.
2
Shenzhen Key Laboratory for Anti-ageing and Regenerative Medicine, Health Science Center, Shenzhen University, Shenzhen, 518060, China.
3
Department of Spinal Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518060, China.
4
State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, 116024, China.
5
Department of Spinal Surgery, Orthopaedic Research Institute, Huangpu Division, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
6
Shenzhen Key Laboratory for Anti-ageing and Regenerative Medicine, Health Science Center, Shenzhen University, Shenzhen, 518060, China. gqzhou@szu.edu.cn.

Abstract

BACKGROUND:

The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA) and induced pluripotent stem cells (iPSCs) are becoming a promising stem cell source.

RESULTS:

Three steps were developed to differentiate human iPSCs into chondrocytes which were transplanted into rat OA models induced by monosodium iodoacetate (MIA). After 6 days embryonic body (EB) formation and 2 weeks differentiation, the gene and protein expression of Col2A1, GAG and Sox9 has significantly increased compare to undifferentiated hiPSCs. After 15 weeks transplantation, no immune responses were observed, micro-CT showed gradual engraftment and the improvement of subchondrol plate integrity, and histological examinations demonstrated articular cartilage matrix production.

CONCLUSIONS:

hiPSC could be an efficient and clinically translatable approach for cartilage tissue regeneration in OA cartilages.

KEYWORDS:

Chondrocyte; Differentiation; Osteoarthritis; Transplantation; iPSC

PMID:
27829414
PMCID:
PMC5103600
DOI:
10.1186/s12896-016-0306-5
[Indexed for MEDLINE]
Free PMC Article

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