Format

Send to

Choose Destination
Oxid Med Cell Longev. 2016;2016:2906953. Epub 2016 Oct 16.

Neurobehavioral and Antioxidant Effects of Ethanolic Extract of Yellow Propolis.

Author information

1
Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, PA, Brazil.
2
Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, PA, Brazil; Programa de Pós-Graduação em Neurociências e Biologia Celular, Belém, PA, Brazil.
3
Departamento de Química, Universidade Estadual do Centro-Oeste (UNICENTRO), 85010-990 Guarapuava, PR, Brazil.
4
Departamento de Química, Universidade Federal de São Carlos (UFSCar), 13565-905 São Carlos, SP, Brazil.
5
Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, PA, Brazil; Programa de Pós-Graduação em Neurociências e Biologia Celular, Belém, PA, Brazil; Laboratório de Farmacologia da Inflamação e do Comportamento, Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará, 66075-900 Belém, PA, Brazil.

Abstract

Propolis is a resin produced by bees from raw material collected from plants, salivary secretions, and beeswax. New therapeutic properties for the Central Nervous System have emerged. We explored the neurobehavioral and antioxidant effects of an ethanolic extract of yellow propolis (EEYP) rich in triterpenoids, primarily lupeol and β-amyrin. Male Wistar rats, 3 months old, were intraperitoneally treated with Tween 5% (control), EEYP (1, 3, 10, and 30 mg/kg), or diazepam, fluoxetine, and caffeine (positive controls) 30 min before the assays. Animals were submitted to open field, elevated plus maze, forced swimming, and inhibitory avoidance tests. After behavioral tasks, blood samples were collected through intracardiac pathway, to evaluate the oxidative balance. The results obtained in the open field and in the elevated plus maze assay showed spontaneous locomotion preserved and anxiolytic-like activity. In the forced swimming test, EEYP demonstrated antidepressant-like activity. In the inhibitory avoidance test, EEYP showed mnemonic activity at 30 mg/kg. In the evaluation of oxidative biochemistry, the extract reduced the production of nitric oxide and malondialdehyde without changing level of total antioxidant, catalase, and superoxide dismutase, induced by behavioral stress. Our results highlight that EEYP emerges as a promising anxiolytic, antidepressant, mnemonic, and antioxidant natural product.

PMID:
27822336
PMCID:
PMC5086397
DOI:
10.1155/2016/2906953
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Hindawi Limited Icon for PubMed Central
Loading ...
Support Center