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Proc Natl Acad Sci U S A. 2016 Nov 22;113(47):13390-13395. Epub 2016 Nov 7.

Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM.

Author information

1
Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, IN 47405.
2
Biology Department, Indiana University, Bloomington, IN 47405.
3
Sir William Dunn School of Pathology, Oxford OX13RE, United Kingdom.
4
Chemistry Department, Indiana University, Bloomington, IN 47405.
5
Max Planck Institute für terrestrische Mikrobiologie, D-35043 Marburg, Germany.
6
Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, IN 47405; stedbell@indiana.edu.

Abstract

The regulated recruitment of Cdc45 and GINS is key to activating the eukaryotic MCM(2-7) replicative helicase. We demonstrate that the homohexameric archaeal MCM helicase associates with orthologs of GINS and Cdc45 in vivo and in vitro. Association of these factors with MCM robustly stimulates the MCM helicase activity. In contrast to the situation in eukaryotes, archaeal Cdc45 and GINS form an extremely stable complex before binding MCM. Further, the archaeal GINS•Cdc45 complex contains two copies of Cdc45. Our analyses give insight into the function and evolution of the conserved core of the archaeal/eukaryotic replisome.

KEYWORDS:

Archaea; CMG; DNA replication; MCM; helicase

PMID:
27821767
PMCID:
PMC5127375
DOI:
10.1073/pnas.1613825113
[Indexed for MEDLINE]
Free PMC Article

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