Format

Send to

Choose Destination
Antimicrob Agents Chemother. 2016 Dec 27;61(1). pii: e02020-16. doi: 10.1128/AAC.02020-16. Print 2017 Jan.

Sustained Nitric Oxide-Releasing Nanoparticles Interfere with Methicillin-Resistant Staphylococcus aureus Adhesion and Biofilm Formation in a Rat Central Venous Catheter Model.

Author information

1
Department of Medicine, Division of Critical Care, St. Anthony Hospital, Oklahoma City, Oklahoma, USA.
2
Department of Medicine, Division of Infectious Diseases, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, New York, USA.
3
Department of Microbiology and Immunology, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, New York, USA.
4
Department of Medicine, Griffin Hospital, Derby, Connecticut, USA.
5
Department of Urology, Albert Einstein College of Medicine, Bronx, New York, USA.
6
Department of Dermatology, George Washington University School of Medicine and Health Sciences, Washington, DC, USA.
7
Department of Medicine, Division of Infectious Diseases, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, New York, USA lmarti13@nyit.edu josh.nosanchuk@einstein.yu.edu.
8
Department of Biomedical Sciences, NYIT College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, New York, USA.

Abstract

Staphylococcus aureus is frequently isolated in the setting of infections of indwelling medical devices, which are mediated by the microbe's ability to form biofilms on a variety of surfaces. Biofilm-embedded bacteria are more resistant to antimicrobial agents than their planktonic counterparts and often cause chronic infections and sepsis, particularly in patients with prolonged hospitalizations. In this study, we demonstrate that sustained nitric oxide-releasing nanoparticles (NO-np) interfere with S. aureus adhesion and prevent biofilm formation on a rat central venous catheter (CVC) model of infection. Confocal and scanning electron microscopy showed that NO-np-treated staphylococcal biofilms displayed considerably reduced thicknesses and bacterial numbers compared to those of control biofilms in vitro and in vivo, respectively. Although both phenotypes, planktonic and biofilm-associated staphylococci, of multiple clinical strains were susceptible to NO-np, bacteria within biofilms were more resistant to killing than their planktonic counterparts. Furthermore, chitosan, a biopolymer found in the exoskeleton of crustaceans and structurally integrated into the nanoparticles, seems to add considerable antimicrobial activity to the technology. Our findings suggest promising development and translational potential of NO-np for use as a prophylactic or therapeutic against bacterial biofilms on CVCs and other medical devices.

KEYWORDS:

Staphylococcus aureus; antimicrobials; biofilms; nanoparticles; nitric oxide

PMID:
27821454
PMCID:
PMC5192117
DOI:
10.1128/AAC.02020-16
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center