Format

Send to

Choose Destination
Nat Chem Biol. 2017 Jan;13(1):9-11. doi: 10.1038/nchembio.2224. Epub 2016 Oct 31.

Multidimensional chemical control of CRISPR-Cas9.

Maji B1,2,3, Moore CL1,4, Zetsche B1,5,6,7, Volz SE1,5,6,7, Zhang F1,5,6,7, Shoulders MD1,4, Choudhary A1,2,3.

Author information

1
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
2
Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
3
Renal Division, Brigham and Women's Hospital, Boston, Massachusetts, USA.
4
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
5
McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
6
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
7
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Abstract

Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation.

PMID:
27820801
PMCID:
PMC5531067
DOI:
10.1038/nchembio.2224
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center