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Nat Chem Biol. 2017 Jan;13(1):15-17. doi: 10.1038/nchembio.2227. Epub 2016 Oct 31.

A multi-step peptidolytic cascade for amino acid recovery in chloroplasts.

Author information

1
Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm, Sweden.
2
Clinical Proteomics Mass Spectrometry, Department of Oncology-Pathology, Science for Life Laboratory and Karolinska Institutet, Stockholm, Sweden.
3
ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia, Australia.
4
Division of Bioorganic Chemistry, Department of Chemistry, Wroclaw University of Technology, Wroclaw, Poland.
5
ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, Victoria, Australia.

Abstract

Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.

PMID:
27820795
DOI:
10.1038/nchembio.2227
[Indexed for MEDLINE]

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