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Ecotoxicology. 2017 Jan;26(1):58-73. doi: 10.1007/s10646-016-1740-9. Epub 2016 Nov 7.

Polyamines-induced aluminum tolerance in mung bean: A study on antioxidant defense and methylglyoxal detoxification systems.

Author information

1
Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan.
2
Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh.
3
Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh.
4
Biomass Chemistry Laboratory, Bioresource Science for Manufacturing, Department of Applied Bioresource Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan.
5
Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan. fujita@ag.kagawa-u.ac.jp.

Abstract

We investigated the roles of exogenously applied Spd (0.3 mM spermidine) in alleviating Al (AlCl3, 0.5 mM, 48 and 72 h)- induced injury in mung bean seedlings (Vigna radiata L. cv. BARI Mung-2). Aluminum toxicity induced oxidative damage overproducing reactive oxygen species (ROS; H2O2 and O2•-), increasing lipoxygenase activity and membrane lipid peroxidation. The toxic compound methylglyoxal (MG) also overproduced under Al stress. In order to circumvent Al-induced oxidative stress, enzymatic and non-enzymatic antioxidant defense were activated by the application of exogenous Spd. Exogenous Spd increased ascorbate (AsA) and glutathione (GSH) content, AsA/dehydroascorbate (DHA) ratio, GSH/ glutathione disulfide (GSSG) ratio, activity of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and catalase (CAT) which reduced ROS production and oxidative stress under Al stress. Spd-induced improvement of GSH pool and Gly II activity alleviated injurious effects of MG. Exogenous Spd positively modulated the endogenous PAs level. Regulating the osmoprotectant molecule (proline), Spd improved plant water status under Al stress. Exogenous Spd was potent to prevent breakdown of Al-induced photosynthetic pigment and to improve growth performances under Al stress. The mechanism by which Spd enhances antioxidant and glyoxalase components might be studied extensively. Spermidine-induced protection of photosynthetic pigment from damages and growth enhancement were remarkable and recommended for further detailed study to understand the mechanism.

KEYWORDS:

Abiotic stress; Acidic soil; Aluminum; Methylglyoxal; Polyamine; ROS signaling

PMID:
27819117
DOI:
10.1007/s10646-016-1740-9
[Indexed for MEDLINE]

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