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Front Microbiol. 2016 Oct 21;7:1623. eCollection 2016.

Immunoprotective Efficacy of Six In vivo-Induced Antigens against Actinobacillus pleuropneumoniae as Potential Vaccine Candidates in Murine Model.

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Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University Chengdu, China.
Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural UniversityChengdu, China; Sichuan Science-observation Experiment of Veterinary Drugs and Veterinary Biological Technology, Ministry of AgricultureChengdu, China.
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca NY, USA.


Six in vivo-induced (IVI) antigens-RnhB, GalU, GalT, Apl_1061, Apl_1166, and HflX were selected for a vaccine trial in a mouse model. The results showed that the IgG levels in each immune group was significantly higher than that of the negative control (P < 0.001). Except rRnhB group, proliferation of splenocytes was observed in all immunized groups and a relatively higher proliferation activity was observed in rGalU and rGalT groups (P < 0.05). In the rGalT vaccinated group, the proportion of CD4+ T cells in spleen was significant higher than that of negative control (P < 0.05). Moreover, proportions of CD4+ T cells in other vaccinated groups were all up-regulated to varying degrees. Up-regulation of both Th1 (IFN-γ, IL-2) and Th2 (IL-4) cytokines were detected. A survival rate of 87.5, 62.5, and 62.5% were obtained among rGalT, rAPL_1166, and rHflX group, respectively while the remaining three groups was only 25%. Histopathological analyses of lungs indicated that surviving animals from the vaccinated groups showed relatively normal pulmonary structure alveoli. These findings confirm that IVI antigens used as vaccine candidates provide partial protection against Actinobacillus pleuropneumoniae infection in a mouse model, which could be used as potential vaccine candidates in piglets.


Actinobacillus pleuropneumoniae; IVI antigens; cellular immune response; humoral immune response; putative vaccine candidates

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