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J Proteomics. 2017 Jan 30;152:206-215. doi: 10.1016/j.jprot.2016.10.020. Epub 2016 Nov 2.

The longissimus thoracis muscle proteome in Alentejana bulls as affected by growth path.

Author information

1
CIISA - Faculdade de Medicina Veterinária, Universidade de Lisboa, ULisboa, Avenida da Universidade Técnica, Pólo Universitário do Alto da Ajuda, 1300-477 Lisboa, Portugal; RUSVM - Ross University School of Veterinary Medicine, PO box 334, Basseterre, St. Kitts and Nevis. Electronic address: adealmeida@rossvet.edu.kn.
2
FGCZ - Functional Genomics Center Zurich, University Zurich/ETH Zurich, Winterthurerstr. 190, CH-8057 Zurich, Switzerland.
3
ITQB/UNL - Instituto de Tecnologia Quimica e Biologica da Universidade Nova de Lisboa, Av. da Republica, 2780-901 Oeiras, Portugal; ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, 7006-554 Évora, Portugal.
4
CIISA - Faculdade de Medicina Veterinária, Universidade de Lisboa, ULisboa, Avenida da Universidade Técnica, Pólo Universitário do Alto da Ajuda, 1300-477 Lisboa, Portugal; L-INIAV Santarém, Instituto Nacional de Investigação Agrária e Veterinária, Quinta da Fonte Boa, 2005-048 Vale de Santarém, Portugal.
5
CIISA - Faculdade de Medicina Veterinária, Universidade de Lisboa, ULisboa, Avenida da Universidade Técnica, Pólo Universitário do Alto da Ajuda, 1300-477 Lisboa, Portugal.

Abstract

Beef production is an important economic activity. In Southern Europe there are two types of beef production systems based on growth paths: continuous (CG) versus discontinuous growth (DG). DG is a traditional system dependent on pasture; whereas in CG animals are supplemented on concentrate feed. We compare the protein abundance profiles of the longissimus thoracis (LT) muscle in CG and DG animals using label-free quantitative proteomics. Twenty three Alentejana male calves (9months-old, 239kg live-weight) were allocated to two feeding regimens. In CG (n=12) production system, animals were fed ad libitum on concentrates plus hay and slaughtered at 18months. In DG (n=11) production system, animals were fed ad libitum on hay from 9 to 15months of age and then the same diet provided to the CG group and slaughtered at 24months. The LT muscle was sampled and protein abundance profiles determined using label-free quantification. We identified 510 proteins, of which 26 showed differential abundance. Several proteins (e.g. Myozenin-2, glyoclythic enzymes and 14-3-3 protein zeta/delta) are proposed as indicators of a more intensive growth path. Myosin binding protein H had higher abundance in the DG group, suggesting it could be associated to discontinuous growth path.

KEYWORDS:

Cattle; Growth path; Longissimus thoracis muscle; Proteome

PMID:
27816513
DOI:
10.1016/j.jprot.2016.10.020
[Indexed for MEDLINE]

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