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Fertil Steril. 2017 Jan;107(1):229-235.e2. doi: 10.1016/j.fertnstert.2016.09.019. Epub 2016 Nov 2.

Type of chromosome abnormality affects embryo morphology dynamics.

Author information

1
IVI Madrid, Madrid, Spain. Electronic address: Mamen.Nogales@ivi.es.
2
IVI Madrid, Madrid, Spain.
3
IGENOMIX, Valencia, Spain.
4
Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain.

Abstract

OBJECTIVE:

To study the differences in the cleavage time between types of embryo chromosomal abnormalities and elaborate algorithm to exclude aneuploid embryos according to the likelihood to be euploid.

DESIGN:

Retrospective cohort study.

SETTING:

University affiliated private center.

PATIENT(S):

Preimplantational genetic screening patients (n = 112) including cases of advanced maternal age, repeated implantation failure, and recurrent miscarriage. A total of 485 embryos were analyzed.

INTERVENTION(S):

None.

MAIN OUTCOME MEASURE(S):

All biopsied embryos were cultured in an incubator with time-lapse technology, cleavage timing from insemination to day 3 and all kinetic parameters that have been described in previous studies by our group.

RESULT(S):

Logistic regression analysis were used to identify morphokinetic parameters and some were strongly associated with complex aneuploid embryos; t3 (odds ratio = 0.590, 95% confidence interval 0.359-0.971) and t5-t2 (odds ratio = 0.151, 95% confidence interval 0.082-0.278).

CONCLUSION(S):

Embryo morphokinetics are affected by chromosome aneuploidy and further analysis of the chromosome content reveals higher differences when the complexity in the chromosome disorders is increased. The use of time-lapse monitoring, although not able to detect an abnormal embryo, may be potentially useful to discard those embryos with high risk of complex chromosomal abnormalities.

KEYWORDS:

Embryo kinetics; array CGH; complex abnormalities; time lapse

[Indexed for MEDLINE]

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