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Methods Mol Biol. 2017;1519:25-41.

Quantitative Efferocytosis Assays.

Author information

1
Department of Microbiology and Immunology and the Centre for Human Immunology, The University of Western Ontario, London, ON, N6A 5C1, Canada.
2
Department of Microbiology and Immunology and the Centre for Human Immunology, The University of Western Ontario, London, ON, N6A 5C1, Canada. bheit@uwo.ca.

Abstract

Efferocytosis, the phagocytic removal of apoptotic cells, is a dynamic process requiring recruitment of numerous regulatory proteins to forming efferosomes in a tightly regulated manner. Herein we describe microscopy-based methods for the enumeration of efferocytic events and characterization of the spatiotemporal dynamics of signaling molecule recruitment to efferosomes, using genetically encoded probes and immunofluorescent labeling. While these methods are illustrated using macrophages, they are applicable to any efferocytic cell type.

KEYWORDS:

Efferocytosis; Immunostaining; Macrophage; Method; Microscopy; Phagocytosis

PMID:
27815871
DOI:
10.1007/978-1-4939-6581-6_3
[Indexed for MEDLINE]

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