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Retrovirology. 2016 Nov 4;13(1):75.

Whole genome sequencing of 51 breast cancers reveals that tumors are devoid of bovine leukemia virus DNA.

Author information

1
Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA), University of Liège (ULg), B34, 1 Avenue de l'Hôpital, 4000, Sart-Tilman Liège, Belgium. n.gillet@ulg.ac.be.
2
Molecular and Cellular Biology, Gembloux Agro-Bio TechUniversity of Liège (ULg), 13 Avenue Maréchal Juin, 5030, Gembloux, Belgium. n.gillet@ulg.ac.be.
3
Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA), University of Liège (ULg), B34, 1 Avenue de l'Hôpital, 4000, Sart-Tilman Liège, Belgium. luc.willems@ulg.ac.be.
4
Molecular and Cellular Biology, Gembloux Agro-Bio TechUniversity of Liège (ULg), 13 Avenue Maréchal Juin, 5030, Gembloux, Belgium. luc.willems@ulg.ac.be.

Abstract

Controversy exists regarding the association of bovine leukemia virus (BLV) and breast cancer. PCR-based experimental evidence indicates that BLV DNA is present in breast tissue and that as many as 37% of cancer cases may be attributable to viral exposure. Since this association might have major consequences for human health, we evaluated 51 whole genomes of breast cancer samples for the presence of BLV DNA. Among 32 billion sequencing reads retrieved from the NCBI database of genotype and phenotype, none mapped on different strains of the BLV genome. Controls for sequence divergence and proviral loads further validated the approach. This unbiased analysis thus excludes a clonal insertion of BLV in breast tumor cells and strongly argues against an association between BLV and breast cancer.

KEYWORDS:

BLV; Bovine leukemia virus; Breast cancer

PMID:
27814725
PMCID:
PMC5095936
DOI:
10.1186/s12977-016-0308-3
[Indexed for MEDLINE]
Free PMC Article

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