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Cytometry A. 2016 Nov;89(11):1017-1030. doi: 10.1002/cyto.a.23016. Epub 2016 Nov 3.

International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices.

Author information

1
Department of Medicine, University of Southern California, Los Angeles, California.
2
Department of Immunology, University of Washington, Seattle, Washington.
3
Flow Cytometry Resource, University of Rochester Medical Center, Rochester, New York.
4
Department of Microbiology and Immunology, University of Texas HSC, San Antonio, Texas.
5
Flow Cytometry Facility, Francis Crick Institute, London, United Kingdom.
6
Division of Rheumatology, Cincinnati Children's Hospital, Cincinnati, Ohio.
7
Flow Cytometry SRL, Instituto Gulbenkian Ciencia, Oeiras, Portugal.
8
New York University Langone Medical Center, Office of Collaborative Science, New York, New York.
9
BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany.
10
University of Virginia School of Medicine, Flow Cytometry Shared Resource Lab, Charlottesville, Virginia.
11
Department of Health Sciences, University of Utah, Salt Lake City, Utah.
12
Garvan Institute of Medical Research, Sydney, Australia.
13
Eli-Lilly, San Diego, California.
14
Flow Cytometry Core Facility, Babraham Institute, Cambridge, United Kingdom.

Abstract

The purpose of this document is to define minimal standards for a flow cytometry shared resource laboratory (SRL) and provide guidance for best practices in several important areas. This effort is driven by the desire of International Society for the Advancement of Cytometry (ISAC) members in SRLs to define and maintain standards of excellence in flow cytometry, and act as a repository for key elements of this information (e.g. example SOPs/training material, etc.). These best practices are not intended to define specifically how to implement these recommendations, but rather to establish minimal goals for an SRL to address in order to achieve excellence. It is hoped that once these best practices are established and implemented they will serve as a template from which similar practices can be defined for other types of SRLs. Identification of the need for best practices first occurred through discussions at the CYTO 2013 SRL Forum, with the most important areas for which best practices should be defined identified through several surveys and SRL track workshops as part of CYTO 2014.

KEYWORDS:

best practices; biosafety; data management; education and training; flow cytometry shared resource; operations management; quality assurance; quality control; reproducibility in science; standard operating procedures

PMID:
27813253
DOI:
10.1002/cyto.a.23016
[Indexed for MEDLINE]
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