Format

Send to

Choose Destination
Methods Mol Biol. 2017;1500:165-184.

Lipid Membrane Encapsulation of a 3D DNA Nano Octahedron.

Perrault SD1,2,3, Shih WM4,5,6.

Author information

1
Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, CLSB 5th Floor, Blackfan Circle, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.
2
Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.
3
Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.
4
Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, CLSB 5th Floor, Blackfan Circle, Boston, MA, 02115, USA.
5
Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA.
6
Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, 02115, USA.

Abstract

Structural DNA nanotechnology methods such as DNA origami allow for the synthesis of highly precise nanometer-scale materials (Rothemund, Nature 440:297-302, 2006; Douglas et al., Nature 459:414-418, 2009). These offer compelling advantages for biomedical applications. Such materials can suffer from structural instability in biological environments due to denaturation and nuclease digestion (Hahn et al., ACS Nano 2014; Perrault and Shih, ACS Nano 8:5132-5140, 2014). Encapsulation of DNA nanostructures in a lipid membrane compartmentalizes them from their environment and prevents denaturation and nuclease digestion (Perrault and Shih, ACS Nano 8:5132-5140, 2014). Here, we describe the encapsulation of a 50 nm DNA nanostructure having the geometry of a wireframe octahedron in a phospholipid membrane containing poly-(ethylene glycol), resulting in biocompatible DNA nanostructures.

KEYWORDS:

DNA; Liposome; NanoOctahedron; Nanotechnology; Vesicle

PMID:
27813008
DOI:
10.1007/978-1-4939-6454-3_12
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center