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Methods Mol Biol. 2017;1500:81-96.

Assembling RNA Nanoparticles.

Author information

1
School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing, 210023, People's Republic of China. sjxiao@nju.edu.cn.

Abstract

RNA nanoparticles are designed and self-assembled according to noncanonical interactions of naturally conserved RNA motifs and/or canonical Watson-Crick base-pairing interactions, which have potential applications in gene therapy and nanomedicine. These artificially engineered nanoparticles are mainly synthesized from in vitro transcribed RNAs, purified by denaturing and native polyacrylamide gel electrophoresis (PAGE), and characterized with native PAGE, AFM, and TEM technologies. The protocols of in vitro transcription, denaturing and native PAGE, and RNA nanoparticle self-assembly are described in detail.

KEYWORDS:

In vitro transcription; Motif; PAGE; RNA nanoparticles; Self-assembly

PMID:
27813002
DOI:
10.1007/978-1-4939-6454-3_6
[Indexed for MEDLINE]

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