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Bull Cancer. 2016 Nov;103(11S):S267-S272. doi: 10.1016/j.bulcan.2016.09.002. Epub 2016 Oct 31.

[Modalities for preparation, cryopreservation, thawing of hematopoietic stem cells and precautions for infusion to patient: Guidelines from the Francophone Society of Bone Marrow Transplantation and Cellular Therapy (SFGM-TC)].

[Article in French]

Author information

1
EFS Nord de France, UTC, 96, rue de Jemmapes, CS 22018, 59013 Lille cedex, France.
2
CHU Nancy, hôpitaux de Brabois, UTC BDT BSP, allée du Morvan, 54500 Vandoeuvre-Lès-Nancy, France.
3
Grand hôpital de Charleroi (GHdC), laboratoire hématologie, 3, Grand'rue, 6000 Charleroi, Belgique.
4
Institut Paoli-Calmettes, centre de thérapie cellulaire, 232, boulevard Ste-Marguerite, BP 156, 13273 Marseille cedex 9, France.
5
CHU-EFS Poitiers, banque de sang placentaire, 350, avenue Jacques-Cœur, 86012 Poitiers cedex, France.
6
CHU Amiens Picardie, laboratoire de thérapie cellulaire, 80054 Amiens cedex 1, France.
7
Hôpital Necker-enfants malades, hématologie, 149, rue de Sèvres, 75015 Paris, France.
8
EFS Bourgogne-Franche-Comte, ICT, BP 1937, 25020 Besançon cedex, France.
9
EFS Bretagne, TC et BSP, BP 91614, 35016 Rennes cedex, France.
10
Hôpitaux universitaires de Genève, hématologie, 4, rue Gabriel-Perret-Gentil, 1205 Genève, Suisse.
11
CHU de Lille, université Lille 2, LIRIC Inserm U995, 59000 Lille, France.
12
CHU Sart-Tilman, hématologie clinique, bâtiment B35, 4000 Liège, Belgique. Electronic address: e.baudoux@chu.ulg.ac.be.

Abstract

To date, despite an existing regulatory framework and standards, there are no true technical recommendations. A survey of 23 cell processing facilities (France, Belgium and Switzerland) has allowed to overview current practices according to cellular products specifications upon arrival at the facility, with modalities for their preparation prior to cryopreservation, storage, thawing and finally for infusion to patient. Data analysis shows great variability of collected volumes and cell concentrations in cellular products. Despite homogeneous practices for handling cells at the facility, methods vary between centers, especially for the choice of cryoprotective solutions and thawing methods. During the workshop, practices have been discussed and summarized to write of recommendations about the following topics: processing and cryopreservation, thawing, bedside precautions (for infusion). This work identifies some improvements in terms of collection, choice of wash solution of thawed cells and validation of the conditions of carriage.

KEYWORDS:

Cellules souches hématopoïétiques; Cryopreservation; Cryopréservation; Décongélation; Hematopoietic stem cells; Thawing

PMID:
27810145
DOI:
10.1016/j.bulcan.2016.09.002
[Indexed for MEDLINE]

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