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BMC Struct Biol. 2016 Nov 3;16(1):19.

Controlled dehydration improves the diffraction quality of two RNA crystals.

Author information

1
X-ray Core Facility, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, Jupiter, FL, 33458, USA. hajpark@scripps.edu.
2
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, Jupiter, FL, 33458, USA.
3
Unit of Polar Genomics, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
4
Department of Polar Sciences, University of Science and Technology, Incheon, 21990, Republic of Korea.
5
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, Jupiter, FL, 33458, USA. disney@scripps.edu.

Abstract

BACKGROUND:

Post-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals.

RESULTS:

We use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders.

CONCLUSION:

The method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structure determinations.

KEYWORDS:

Crystal dehydration; Free mounting system; Post-crystallization modification; RNA; Repeat expansion

PMID:
27809904
PMCID:
PMC5093936
DOI:
10.1186/s12900-016-0069-1
[Indexed for MEDLINE]
Free PMC Article

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