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Mol Biol Cell. 2017 Jan 1;28(1):21-29. doi: 10.1091/mbc.E16-09-0659. Epub 2016 Nov 2.

The p150N domain of chromatin assembly factor-1 regulates Ki-67 accumulation on the mitotic perichromosomal layer.

Author information

  • 1Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605.
  • 2Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605 paul.kaufman1@umassmed.edu.

Abstract

Chromatin assembly factor 1 (CAF-1) deposits histones during DNA synthesis. The p150 subunit of human CAF-1 contains an N-terminal domain (p150N) that is dispensable for histone deposition but promotes the localization of specific loci (nucleolar-associated domains [NADs]) and proteins to the nucleolus during interphase. One of the p150N-regulated proteins is proliferation antigen Ki-67, whose depletion also decreases the nucleolar association of NADs. Ki-67 is also a fundamental component of the perichromosomal layer (PCL), a sheath of proteins surrounding condensed chromosomes during mitosis. We show here that a subset of p150 localizes to the PCL during mitosis and that p150N is required for normal levels of Ki-67 accumulation on the PCL. This activity requires the sumoylation-interacting motif within p150N, which is also required for the nucleolar localization of NADs and Ki-67 during interphase. In this manner, p150N coordinates both interphase and mitotic nuclear structures via Ki67.

PMID:
27807046
PMCID:
PMC5221625
[Available on 2017-03-16]
DOI:
10.1091/mbc.E16-09-0659
[PubMed - in process]
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