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BMC Genomics. 2016 Nov 2;17(1):853.

Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants.

Author information

1
Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, Universitätsstrasse 31, 93053, Regensburg, Germany.
2
Department of Statistical Bioinformatics, University of Regensburg, Universitätsstrasse 31, 93053, Regensburg, Germany.
3
Present address: The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
4
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076, Tübingen, Germany.
5
Present address: Intomics A/S, Diplomvej 377, 2800, Lyngby, Denmark.
6
Biochemistry Center Regensburg (BZR), Biochemistry II, University of Regensburg, Universitätsstrasse 31, 93053, Regensburg, Germany.
7
European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV, Groningen, The Netherlands.
8
Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, Universitätsstrasse 31, 93053, Regensburg, Germany. gunter.meister@vkl.uni-regensburg.de.

Abstract

BACKGROUND:

Volvox carteri (V. carteri) is a multicellular green alga used as model system for the evolution of multicellularity. So far, the contribution of small RNA pathways to these phenomena is not understood. Thus, we have sequenced V. carteri Argonaute 3 (VcAGO3)-associated small RNAs from different developmental stages.

RESULTS:

Using this functional approach, we define the Volvox microRNA (miRNA) repertoire and show that miRNAs are not conserved in the closely related unicellular alga Chlamydomonas reinhardtii. Furthermore, we find that miRNAs are differentially expressed during different life stages of V. carteri. In addition to miRNAs, transposon-associated small RNAs or phased siRNA loci, which are common in higher land plants, are highly abundant in Volvox as well. Transposons not only give rise to miRNAs and other small RNAs, they are also targets of small RNAs.

CONCLUSION:

Our analyses reveal a surprisingly complex small RNA network in Volvox as elaborate as in higher land plants. At least the identified VcAGO3-associated miRNAs are not conserved in C. reinhardtii suggesting fast evolution of small RNA systems. Thus, distinct small RNAs may contribute to multicellularity and also division of labor in reproductive and somatic cells.

KEYWORDS:

Argonaute; Evolution; Gene silencing; Small RNAs; Transposons; Volvox carteri; microRNAs

PMID:
27806710
PMCID:
PMC5093975
DOI:
10.1186/s12864-016-3202-4
[Indexed for MEDLINE]
Free PMC Article

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