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Cell Rep. 2016 Nov 1;17(6):1505-1517. doi: 10.1016/j.celrep.2016.10.018.

Role for the IFT-A Complex in Selective Transport to the Primary Cilium.

Author information

1
Department of Pathology and Perlmutter Cancer Center, NYU School of Medicine, Smilow Research Building, 522 First Avenue, New York, NY 10016, USA.
2
Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA; Broad Institute, Cambridge, MA 02142, USA.
3
Department of Pathology and Perlmutter Cancer Center, NYU School of Medicine, Smilow Research Building, 522 First Avenue, New York, NY 10016, USA. Electronic address: brian.dynlacht@nyumc.org.

Abstract

Intraflagellar transport sub-complex A (IFT-A) is known to regulate retrograde IFT in the cilium. To rigorously assess its other possible roles, we knocked out an IFT-A subunit, IFT121/WDR35, in mammalian cells and screened the localization of more than 50 proteins. We found that Wdr35 regulates cilium assembly by selectively regulating transport of distinct cargoes. Beyond its role in retrograde transport, we show that Wdr35 functions in fusion of Rab8 vesicles at the nascent cilium, protein exit from the cilium, and centriolar satellite organization. Furthermore, we show that Wdr35 is essential for entry of many membrane proteins into the cilium through robust interactions with cargoes and other IFT-A subunits, but the actin network functions to dampen this transport. Wdr35 is mutated in several ciliopathies, and we find that certain disease mutations impair interactions with cargo and other IFT-A subunits. Together, our data link defects in IFT-A mediated cargo transport with disease.

KEYWORDS:

Arl13b; IFT-A; WDR35; actin; cargo transport; centrosome; cilia; ciliary membrane; cilium

PMID:
27806291
PMCID:
PMC5123888
DOI:
10.1016/j.celrep.2016.10.018
[Indexed for MEDLINE]
Free PMC Article

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