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Cell Rep. 2016 Nov 1;17(6):1473-1481. doi: 10.1016/j.celrep.2016.10.016.

Onset of Spinal Cord Astrocyte Precursor Emigration from the Ventricular Zone Involves the Zeb1 Transcription Factor.

Author information

1
INSERM U1051, Institut des Neurosciences de Montpellier, 34091 Montpellier, France; Université de Toulouse, Centre National de la Recherche Scientifique (CNRS), CBD-UMR5547, 31062 Toulouse, France; Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK.
2
INSERM U1051, Institut des Neurosciences de Montpellier, 34091 Montpellier, France.
3
INSERM U1051, Institut des Neurosciences de Montpellier, 34091 Montpellier, France; Department of Animal Biology, Faculty of Sciences, Damascus University, Damascus, Syria.
4
Department of Perinatology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi 480-0392, Japan.
5
Université de Toulouse, Centre National de la Recherche Scientifique (CNRS), CBD-UMR5547, 31062 Toulouse, France.
6
Department of Oral Immunology and Infectious Diseases, University of Louisville, Louisville, KY 40202, USA.
7
PhyMedExp, INSERM U1046, CNRS UMR 9214, University of Montpellier, 34295 Montpellier, France.
8
Institut für Biochemie, Emil-Fischer-Zentrum, Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
9
Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK.
10
INSERM U1051, Institut des Neurosciences de Montpellier, 34091 Montpellier, France. Electronic address: alexandre.pattyn@inserm.fr.

Abstract

During spinal cord development, astrocyte precursors arise from neuroepithelial progenitors, delaminate from the ventricular zone, and migrate toward their final locations where they differentiate. Although the mechanisms underlying their early specification and late differentiation are being deciphered, less is known about the temporal control of their migration. Here, we show that the epithelial-mesenchymal transition regulator Zeb1 is expressed in glial precursors and report that loss of Zeb1 function specifically delays the onset of astrocyte precursor delamination from the ventricular zone, correlating with transient deregulation of the adhesion protein Cadherin-1. Consequently, astrocyte precursor invasion into the Zeb1-/- mutant white matter is delayed, and induction of their differentiation is postponed. These findings illustrate how fine regulation of adhesive properties influences the onset of neural precursor migration and further support the notion that duration of exposure of migrating astrocyte precursors to environmental cues and/or their correct positioning influence the timing of their differentiation.

KEYWORDS:

Cadherin-1; GFAP; Sox9; Zeb1; Zeb2; astrocyte; cell migration; development; oligodendrocyte; spinal cord

PMID:
27806288
DOI:
10.1016/j.celrep.2016.10.016
[Indexed for MEDLINE]
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