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Mol Nutr Food Res. 2017 Apr;61(4). doi: 10.1002/mnfr.201600169. Epub 2016 Nov 30.

Pomegranate extract prevents skeletal muscle of mice against wasting induced by acute TNF-α injection.

Author information

1
Institute of Neuroscience, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
2
Institut Meurice, Haute Ecole Lucia de Brouckère, Anderlecht, Belgium.
3
Department of BioMolecular Sciences, Division of Pharmacognosy, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, MS, USA.

Abstract

SCOPE:

We investigated whether punicalagin-rich pomegranate extract (PE) protects skeletal muscle of mice against inflammation induced by an acute injection of TNF-α.

RESULTS:

Mice fed with PE or standard chow during 6 wk were injected with TNF-α (100 ng/g) or vehicle and sacrificed 6 h later. Prior supplementation with PE prevented the loss of tibialis anterior mass induced by TNF-α. In skeletal muscle, the activation of the NF-κB signaling and the induction of cytokines mRNA were reduced in mice having received PE. In those mice, the activity of the Akt/mTORC1 pathway and the protein synthesis were maintained after TNF-α injection whereas markers involved in the ubiquitin proteasome pathway were less activated. As urolithin A was the only punicalagin metabolite detectable in plasma of mice supplemented with PE, we performed in vitro experiments using a murine cell line (C2C12) to provide evidence that urolithin A is likely the active compound protecting skeletal muscle against TNF-α-induced inflammation.

CONCLUSION (FOCUS ON NUTRITIONAL RELEVANCE):

These results suggest that supplementation with a punicalagin-rich PE may protect skeletal muscle against an acute inflammation.

KEYWORDS:

Ellagitannins; NF-κB; Polyphenols; Punicalagins; Urolithin A

PMID:
27804206
DOI:
10.1002/mnfr.201600169
[Indexed for MEDLINE]

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